Description
This Mouse CD300 Molecule Like Family Member B (CD300LB) ELISA Kit is intended for quantitative detection of mouse CD300b in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse CD300b ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD300b has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: I18-P209) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD300b is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD300b amount of sample captured in plate.
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: CD300 molecule like family member b is a protein that in humans is encoded by the CD300LB gene. It is mapped to 11; 11 E2. CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells